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KMID : 1098419940020010044
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Korean Journal of Medicinal Crop Science
1994 Volume.2 No. 1 p.44 ~ p.50
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Effect of Carbon and Nitrogen Source on Somatic Embryogenesis in Suspension Culture of Ligusticum chuanxiang Hort
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채영암/Chae, Young Am
박상언/Park, Sang Un
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Abstract
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This study was carried out to select the appropriate medium(especially, carbon and nitrogen source ) for somatic embryogenesis in order to develop the rapid mass production system in suspension culture of chuanxiang Hort. Suitable medium for somatic embryo formation was MS medium. The half strength MS medium was effective for somatic embryo development. Sucrose was the most effective carbon source for somatic embryo formation, however, production of somatic embryos was reduced at higher concentration of sucrose. Effects of suger was the same as sucrose. Somatic embryo formation was higher as the decrease of $NH_{4}NO_3$ 수식 이미지, and optimum ratio of $KNO_3\;:\;NH_4NO_3$ 수식 이미지 was 825 : 238mg /1. Regenerated plant was obtained in MS basal medium and survival late of plantlet was 60-70% after transplanted directly to the vermiculite.
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